| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.015488 |
| Chromosome: | chromosome 1 |
| Location: | 6766215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048350 | EBM1,CGL53 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); conserved protein with glycosyl hydrolase family 5 domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCGGCAGTTAGCAAGACAAGCATTTGTCGCGACGACGGGTCACGCAG |
| Internal bar code: | AGCGTTGGTCCTCAGTTTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAACCTCACAGTCCTCC |
| Suggested primer 2: | CTAGCTCCCAGCCCATGATG |