| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.015490 |
| Chromosome: | chromosome 6 |
| Location: | 3729634 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278161 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCATGTGAAAGCCTTACCCGGGGGCATGTGCCCGAGCTCGACGGGTT |
| Internal bar code: | ACAAAGTTGCGTCTGAGGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1313 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACATGGACAGACACGG |
| Suggested primer 2: | TTTCCTTTTACAAGCGCCGC |