Insertion junction: CLIP2.015496_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.015496
Chromosome:chromosome 16
Location:1185809
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g650600 MST1 Mastigoneme-like flagellar protein; (1 of 4) IPR009030//IPR011641 - Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACCGTTGAGCTGGCATACTCGAAGACAAAAAATTTGGGTGAGAAAGG
Internal bar code:CTTTGGTCGTATAAGAGAGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4676
LEAP-Seq percent confirming:95.9184
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACGCCTTACACGAACAT
Suggested primer 2:AGCCACAAACCTTACCCGAG