Insertion junction: CLIP2.015513_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.015513
Chromosome:chromosome 17
Location:1986493
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g710600 (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGTCATGAGGGCGCTGGTCAACTCGAACGAATCCCAGTCCACCAGCGC
Internal bar code:TAACAGTTTAGTAATCATTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2661
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGCGTTTTAGGCTGTATG
Suggested primer 2:GCACGTACACTCCAGAACCA