| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.015551 |
| Chromosome: | chromosome 1 |
| Location: | 1765486 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009731 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGACATGGCTGGAGGTCTGCAGGGCGATTGTGGCGGAGCCGGGATG |
| Internal bar code: | TCGGTTGGAGTTCAGATGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGTGCTGGTGTTGGT |
| Suggested primer 2: | GACCGACTCTTGCACTGACA |