Insertion junction: CLIP2.015553_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.015553
Chromosome:chromosome 2
Location:2722185
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAGGCCGCCGCGGGCGGTGAGGCCGTGCCGGTCGAGCACCAGGGTGC
Internal bar code:TATGTTTGTGCCCTGTCGCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2711
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:99
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:99

Suggested primers for confirmation by PCR

Suggested primer 1:GTCATTCCCGAACCAGACGT
Suggested primer 2:CCAAAGTCCAGGGCAGACAT