Insertion junction: CLIP2.015564_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.015564
Chromosome:chromosome 5
Location:2757896
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g240700 FAP378 Flagellar Associated Protein 378; (1 of 1) PF00530//PF05572 - Scavenger receptor cysteine-rich domain (SRCR) // Pregnancy-associated plasma protein-A (Peptidase_M43) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACGATCGGTGAGACGGGAGCTGAGGGAGA
Internal bar code:GGGGACGTCTGTGGCGGGCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:625
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTAGCACATACCGGTACC
Suggested primer 2:TTACAGAGCTCCGCGTTTGT