Insertion junction: CLIP2.015605_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015605
Chromosome:chromosome 17
Location:1146507
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802033 CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCTCTCGCCCATCCACCAGGCTGCGGTAGGTGTTGTGCAGTTCGTCG
Internal bar code:GATCCGTTCACATACATCGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:675
LEAP-Seq percent confirming:27.2727
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCCTCGTTCTCCATCTC
Suggested primer 2:CATCCATCTCGTCCAGCTCC