| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.015611 |
| Chromosome: | chromosome 2 |
| Location: | 4700429 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108100 | (1 of 1) PTHR12147//PTHR12147:SF22 - FXNA-RELATED // ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCACAGCTCCCAGAATGGAGACTCCGATTCATGGCTGAAACGCACCA |
| Internal bar code: | GATCTGAGTGACGCCGAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTTCTCTAGGCACGCT |
| Suggested primer 2: | CCACACGAAGCAACACTGTG |