| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015628 |
| Chromosome: | chromosome 8 |
| Location: | 65747 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358521 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGTAGCACCCATGACTGCCGCCGCCTCTGCCACACCCAGCAGTGCG |
| Internal bar code: | CGAAAGTACATGAGCGTCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3948 |
| LEAP-Seq percent confirming: | 96.4912 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTAGCCGTGGAGCCTATG |
| Suggested primer 2: | CCACCGCGCTTGTTTTTACA |