| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015632 |
| Chromosome: | chromosome 10 |
| Location: | 3066078 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441100 | PIGT,GPIT1,GPI16,GIT1,PIGT1 | GPI transamidase component Gpi16; (1 of 1) K05292 - phosphatidylinositol glycan, class T (PIGT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAATGGCTTGGTGGGTGGGTTGGCAAAAGGGAAGGCTGTTTCACAGTT |
| Internal bar code: | CGTATTGACTCCAACCGTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3335 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCTCACAGTCACGGACGG |
| Suggested primer 2: | ATGCCATGCCGTTATCCGAT |