Insertion junction: CLIP2.015637_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.015637
Chromosome:chromosome 5
Location:1204091
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g241631 (1 of 1) IPR000253//IPR002893 - Forkhead-associated (FHA) domain // Zinc finger, MYND-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACGCATCGACTAACAAACGGATAAAAGTGCACCGCGTCCAGCGGCAC
Internal bar code:ATGGTATAACGGGGGGTAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:783
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCATAAAATGCCTCCGC
Suggested primer 2:ATCACACAGCTGTCGACCAG