| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015639 |
| Chromosome: | chromosome 16 |
| Location: | 7066456 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674852 | HAP2,GCS1 | (1 of 1) PF10699 - Male gamete fusion factor (HAP2-GCS1); Generative cell specific-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCGCGGCTTAGTGCTATGTTTGCCCTTTTCCTTGTCCACTTCCTTC |
| Internal bar code: | CCACGTTTTCGGTGTTGGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1889 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTATGTGAAGTGTGGCA |
| Suggested primer 2: | CATACTGATTGGCCGCAAGC |