Insertion junction: CLIP2.015645_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.015645
Chromosome:chromosome 8
Location:3823509
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381450 OPR35 OctotricoPeptide Repeat protein 35 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATGGGTAGGGGGAGCTGGAACTGGCCGGACGATGAATACTTGGCCTCG
Internal bar code:TGGACGGTTGCCGGGTACTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:197
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCGCCTGCTATACTACC
Suggested primer 2:CCTTTATCGTTTCGGCGCTG