Insertion junction: CLIP2.015661_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.015661
Chromosome:chromosome 16
Location:4261654
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g669500 (1 of 726) IPR011009 - Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCAACCAGTCGCTCGATCGCACCCGCGCACCCTTCACGCCTGCTTTCG
Internal bar code:ACATTCACGGCAATAGGACAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2498
LEAP-Seq percent confirming:94.3396
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGGGACGCAATCGGTAT
Suggested primer 2:CAATGTTTGCTCAGGTGGGC