| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015717 |
| Chromosome: | chromosome 2 |
| Location: | 7728551 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCTGCCTAAACAACACCTGTAATTCTTGCCCCTGCAGAAAGCGGCGGC |
| Internal bar code: | ACGAGCCCGGAGCTAACTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2488 |
| LEAP-Seq percent confirming: | 26.2295 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCACACAGGCAAACACG |
| Suggested primer 2: | GATGGGGCCCGAGTATGTG |