| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.015750 |
| Chromosome: | chromosome 17 |
| Location: | 4396027 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730600 | (1 of 6) 3.2.1.4 - Cellulase / Endoglucanase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCGCGTTCCAGTCACAGGGGGCGGCGTAGTTGGTGGCGCAGCTGCTG |
| Internal bar code: | TACCGTACATGAAAGGCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5757 |
| LEAP-Seq percent confirming: | 73.8462 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGGTGTATTCGGTCGGC |
| Suggested primer 2: | GTGCGTGTGTGTGTGTGTAC |