| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015756 |
| Chromosome: | chromosome 7 |
| Location: | 1932837 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325749 | (1 of 1) PF14924 - Protein of unknown function (DUF4497) (DUF4497) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTACCGCAATACGGTACTCACGGGCTTTGCGTGGCGAGGTTGTGCCTG |
| Internal bar code: | AGGATGAGTGGGGCGATTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAAGGCCCCTTTCCCAA |
| Suggested primer 2: | GCACACTACTCTTCCCACCC |