| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.015791 |
| Chromosome: | chromosome 7 |
| Location: | 5266869 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349520 | (1 of 1) PF00023//PF00069//PF13857 - Ankyrin repeat (Ank) // Protein kinase domain (Pkinase) // Ankyrin repeats (many copies) (Ank_5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCGAGGCACACATACACGTAGGAGAGCACGCGCATGGAGGCTCATCC |
| Internal bar code: | GTATGGATAGTGAGGGTAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTATTAGCCACGCCCA |
| Suggested primer 2: | AGTAGTGGCACGAACCTTGG |