| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015814 |
| Chromosome: | chromosome 12 |
| Location: | 1868889 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512200 | MFT26 | Major facilitator superfamily transporter; (1 of 1) PTHR23504//PTHR23504:SF15 - FAMILY NOT NAMED // PROTEIN ZINC INDUCED FACILITATOR 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGTTAGAAAGCCGCTGAAGCTGAAGATACCGGCCTGACAAAAAAATA |
| Internal bar code: | CGAAGAACAACTGGCGCGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3458 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 121 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATGGTCCGCGACTTTGA |
| Suggested primer 2: | AGAGGGAGCAGTGGAGAGAG |