| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015817 |
| Chromosome: | chromosome 10 |
| Location: | 1118494 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425450 | AOF5 | (1 of 1) 1.5.99.4 - Nicotine dehydrogenase; Flavin-containing amine oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGCGCGCCGTCGAAGGGCAGGTCTGCGCGGGTGGGGTCGGGGGGTCC |
| Internal bar code: | TGCTAGGTCGGTTCACGTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTCTCGCACCTTTGAC |
| Suggested primer 2: | CCATGCACCCAACGTTTGTT |