Insertion junction: CLIP2.015824_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.015824
Chromosome:chromosome 4
Location:1277235
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g214800 VMPL1,VMP6 (1 of 10) PF00957 - Synaptobrevin (Synaptobrevin); Endosomal R-SNARE protein, VAMP-like family (R.III) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGACCGCGCGCAATGTGTCCCTGACACCATCCCGCACCACCCCGCCC
Internal bar code:GGGTCTGGTGCATGCCTACCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3593
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGTTCCGCATGGACTCT
Suggested primer 2:CGCACCTTCAACGTCAAGTG