Insertion junction: CLIP2.015832_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.015832
Chromosome:chromosome 3
Location:2296436
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g157751 (1 of 1) IPR011047//IPR018391//IPR027295 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat // Quinonprotein alcohol dehydrogenase-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGTCGCCCGGTGCCTGTGACTGCCTCCCCACCTCCATCGGTTACTCCC
Internal bar code:AAATTTGTCAAGGGTTTAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4111
LEAP-Seq percent confirming:14.8936
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAGGCGCTTCTTTTCGA
Suggested primer 2:GTGTCTTCTGCATCCAGCCT