Insertion junction: CLIP2.015844_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.015844
Chromosome:chromosome 1
Location:5628328
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040200 WNK2 (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK); WNK protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGAGAAGGTGGACGTGTACTCGTTCGGGTGGGTGGCCGAGTGGTGGGC
Internal bar code:CTCTTCTCGCAGACTTTGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:5117
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCTGTCGCTCATGTGTT
Suggested primer 2:ACAGAGCTGTTTACGTCCGG