| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015845 |
| Chromosome: | chromosome 13 |
| Location: | 2869477 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583150 | (1 of 2) IPR000104//IPR000210//IPR011333 - Antifreeze protein, type I // BTB/POZ domain // POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTGCAGCACCCACACCAGCCGCGGCCGCACGAGCTGCGGCCGCTGC |
| Internal bar code: | TTACTTATTTGTTACGTGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTAGGCTACCACGTGC |
| Suggested primer 2: | GTGGACTGGGAAGACAGACG |