Insertion junction: CLIP2.015862_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.015862
Chromosome:chromosome 3
Location:6674497
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g195366 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCGTGTTGGTTTCGACAACAGGGTCTGCGGCTGGCACGCGCCCCGGC
Internal bar code:TCGTAGTGCCACCCAAGATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1449
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGGACTGATAGCCAGCA
Suggested primer 2:CGCATCAAAGTATGGCCGTG