| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.015881 |
| Chromosome: | chromosome 5 |
| Location: | 3177838 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232050 | (1 of 37) IPR008979 - Galactose-binding domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGATGAGACATACGGTAGTTAGGCGGCGGCATGGTAAGTAGGATGATA |
| Internal bar code: | TACTCTAAAAGTCTAGTGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1410 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGATGGATGGATGTCGCC |
| Suggested primer 2: | ACATTGCGGAGAGAAGTGCA |