Insertion junction: CLIP2.015895_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015895
Chromosome:chromosome 12
Location:5332987
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g528600 TNP31,CSB47 {"Probable transposon-derived protein of Chlamydomonas-Specific family B; (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCTCATAAGGAATCGGCCCCGCTTCTGGCGCCGCACACGTAAACATA
Internal bar code:CGCGTATAGACAGTTGTATTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:708
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGCATGATTAAGGGCG
Suggested primer 2:GTTCTGGGGATGGGGAACAG