Insertion junction: CLIP2.015898_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015898
Chromosome:chromosome 1
Location:1805623
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g010100 NAT7 N-acetyltransferase; (1 of 2) 2.3.1.128 - Ribosomal-protein-alanine N-acetyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCGGGCCCAGCACACATGACGCATGTGAATCCCCGCTCCTGCACCCCG
Internal bar code:ATGTTTCGGCGAAGAAACACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2390
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACGTTTGCCTTTTGGGG
Suggested primer 2:TGATTCTGTGTGTCAGCGCT