Insertion junction: CLIP2.015909_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015909
Chromosome:chromosome 17
Location:6800283
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g746897 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCAGGGAGGGCTGAGTGGATGCGTGCCGCTGCCAGCGCCAGTGCCAGT
Internal bar code:TGAGCTATATATCCGTAATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCCCATCCATAACAACCC
Suggested primer 2:CTGGGTCTCCGACGTTTGAA