Insertion junction: CLIP2.015937_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015937
Chromosome:chromosome 7
Location:837980
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g318400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGATACGTTCTGGACTTGTGGAGGGGTATGTGGCGTGGAGGAAGAGAG
Internal bar code:TGCGTTCAGTATCGTAGATCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1937
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:GCACACACTTGTCAACCGTC
Suggested primer 2:AACCCTCCCTCCCCTATCAC