Insertion junction: CLIP2.015940_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.015940
Chromosome:chromosome 6
Location:2282440
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g267250 MKP2 (1 of 3) K04459 - dual specificity MAP kinase phosphatase (DUSP, MKP); Dual-specificity protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTACGGATGGAAAGGCGCTTGGAGGGCATGTGCATGGGCTGAGCGGCCC
Internal bar code:AAAAGCTGGAGTTTTCTGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3344
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGACTCCATGATCAGTCG
Suggested primer 2:TGTGTGTTTCCCCAGCAGAG