| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.015945 |
| Chromosome: | chromosome 17 |
| Location: | 4945527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735450 | SUB14 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCAAAGTCATACCAGCCTGCGGGAAGGGAGCGGGGCCGAGGGCGGGTT |
| Internal bar code: | CATAGCAAATTGACGATTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACTACTACCGCCTGAGT |
| Suggested primer 2: | GTCACATTTGCAACAGCCGT |