| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.015946 |
| Chromosome: | chromosome 6 |
| Location: | 2718844 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270900 | SUV3 | Mitochondrial DEAD-box DNA/RNA helicase; (1 of 1) K17675 - ATP-dependent RNA helicase SUPV3L1/SUV3 [EC:3.6.4.13] (SUPV3L1, SUV3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCACGCGCAGGGAACGCTACAACTTCCGCCGGGACGGCGGCATTCCC |
| Internal bar code: | GTAAGTATAGGTGTTCACTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4521 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCGTTCTGTCAACTCTCC |
| Suggested primer 2: | GCGATAGTTAGGCAGCTCGT |