| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.015951 |
| Chromosome: | chromosome 12 |
| Location: | 1752053 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486050 | (1 of 1) PF00098//PF00397 - Zinc knuckle (zf-CCHC) // WW domain (WW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGGGCACCCAGTTCAAATCGTGGGGCGCGGCTGCCGAAGTCTTTCACA |
| Internal bar code: | GCTGCAGAATTATAGTTTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1321 |
| LEAP-Seq percent confirming: | 32.1429 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTCGCCGTCTAAGTATG |
| Suggested primer 2: | GTTCAGGCGAACCCAAGAGA |