| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.015952 |
| Chromosome: | chromosome 5 |
| Location: | 1924725 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236050 | (1 of 2) IPR015097 - Lung surfactant protein D coiled-coil trimerisation | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTGGAGGGCCGCGAGCGGCGGTCAGATGAGGCTGCTCGCGCGGCACA |
| Internal bar code: | AGGATAGACGTTGTGGTTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3247 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTACGGTGTAGGCGATG |
| Suggested primer 2: | GACTTCTCAGCGGGTGTGAA |