| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.015957 |
| Chromosome: | chromosome 10 |
| Location: | 5662322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459050 | (1 of 4) 3.4.21.83 - Oligopeptidase B / Protease II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCAGGGCGGAGGCCAGGGAGCGGAGGGGGGTGAGGGCGAAGCGGTC |
| Internal bar code: | CATGGATTTATAGGACTTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1810 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGAAGGCAGTTTCTGGAT |
| Suggested primer 2: | CTGCAGCTCTCACATCCTGT |