Insertion junction: CLIP2.015995_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.015995
Chromosome:chromosome 13
Location:4186387
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g591450 (1 of 2) PTHR11767//PTHR11767:SF47 - INWARD RECTIFIER POTASSIUM CHANNEL // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGAGCTAGGCCGTGGTAGAGGCGCGCGCATCAGAGGGCCGCCCTGCCT
Internal bar code:AGGGTCAGTCAGTACCGAAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAAGAACGGCTAGCCCAG
Suggested primer 2:GCTCTGTGTCCCTACCCCTA