| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.015996 |
| Chromosome: | chromosome 16 |
| Location: | 1870178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTCCAGCCCGGGCGCCGGCTGCGACACCGGCTGCGGCTGTAGCACT |
| Internal bar code: | ACCTACCAGTAAGTGAACACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACCATCACCAAGGCAT |
| Suggested primer 2: | GCTTGTTATGGCGGTTGTGG |