Insertion junction: CLIP2.016006_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016006
Chromosome:chromosome 6
Location:5180226
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g283550 CRC2,POC6 Proteome of centriole protein 6; (1 of 1) K16466 - centrin-3 (CETN3, CDC31) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGGAGAGGAGAGCACGCAACAACTGGACTTCCAGGTGTGGTGTTGGC
Internal bar code:CTCGCGCGCCACATTCATCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1881
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCGTTGCAAGTACGAGA
Suggested primer 2:CAAATACCGTGCCAGACCCT