| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.016032 |
| Chromosome: | chromosome 12 |
| Location: | 5875215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATCCTCCCACGCGGAGTCAGGCGAGTATGGACATAACAGGAGCAGGGG |
| Internal bar code: | TTTGTTATAATCTCCAAATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2774 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 110 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCTTCGCTGAGCAGGAG |
| Suggested primer 2: | CAAGTGAGTGTTTCAGCGGC |