Insertion junction: CLIP2.016036_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016036
Chromosome:chromosome 12
Location:1567753
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g487400 (1 of 2) PF03556//PF14555 - Cullin binding (Cullin_binding) // UBA-like domain (UBA_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGCGATCGAAGCACCGACAGCCTACTACCATACACATACACACAAGC
Internal bar code:TTTTTATAAAGCGCCTAATCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:606
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCTTGTTTCCAACTGGG
Suggested primer 2:CACGCAACAGACACAAGGTG