Insertion junction: CLIP2.016052_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016052
Chromosome:chromosome 10
Location:4060976
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g447850 YEE2 (1 of 3) K07112 - uncharacterized protein (K07112); Integral membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTATGTATACCCCCACCACCAACCCCCGTGCTCAGCGCAATCGCCGTG
Internal bar code:GTGTTTGAAGGGTTCTTGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2055
LEAP-Seq percent confirming:92.6829
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTCGGAGTTACGACGTC
Suggested primer 2:CCTGAGCGCATCCCTAGAAG