Insertion junction: CLIP2.016054_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.016054
Chromosome:chromosome 13
Location:1243229
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g570400 (1 of 3) 3.1.1.14 - Chlorophyllase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTTGAGGCTGTAGCTCAGGGGTGCCAGGCGCTCGGCGGCGGCGGCTG
Internal bar code:CTAATCTGGGTAACTCTCTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:338
LEAP-Seq percent confirming:58.8235
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATGCATGTGTGTTGGTG
Suggested primer 2:CAGTCACTGCTTGGCTCTGA