| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.016062 |
| Chromosome: | chromosome 12 |
| Location: | 2009168 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801373 | (1 of 2) IPR000104//IPR000477//IPR005135 - Antifreeze protein, type I // Reverse transcriptase domain // Endonuclease/exonuclease/phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTCGTAGCTGACTTCTGGGGTCGTCTCCAGACGTTTGTGACTTTGGG |
| Internal bar code: | AGGGTGTCTAGGTGTCGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4209 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTTTGCTGCAAACCCTC |
| Suggested primer 2: | ATGTGACTGCTGTGTGGAGG |