| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.016086 |
| Chromosome: | chromosome 9 |
| Location: | 1296881 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400750 | AMT5,AMT1Ea,AMT1E | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGTCTCGTTTCCCTTCCACACAGATACTCGCACCGGCGCTATATGAA |
| Internal bar code: | GATCGTACCCTTATCATGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTTGGGAACACACTTTG |
| Suggested primer 2: | ACCTGCTGTCTACCTCGCTA |