| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.016109 |
| Chromosome: | chromosome 11 |
| Location: | 194722 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467549 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGAGATGGCGCAAGCACTGCCGGCTCTGGTGCTCACGCCGCCCGCG |
| Internal bar code: | CGTTTTTTCACGTCAGAAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1096 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATGGATGGGTATCTCGG |
| Suggested primer 2: | ACCATGCCACAGCTGCTTAT |