Insertion junction: CLIP2.016113_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.016113
Chromosome:chromosome 10
Location:2086243
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g432900 AKR3 Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF170 - ALDO/KETO REDUCTASE // ALDOSE REDUCTASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCAACAGTGCAGCAAGATCCACGGATGATGAAGGAAGTCCTACTTA
Internal bar code:GAGGTCGGATGGTGCGGGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:858
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGACCTCCCAGATGCTGATC
Suggested primer 2:GTCGTGCTCACCACCTTGTA