Insertion junction: CLIP2.016114_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016114
Chromosome:chromosome 10
Location:397571
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g420550 TSP4,CTPA4,CTPA1 (1 of 1) IPR004447 - C-terminal-processing peptidase S41A; C-terminal peptidase A intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAGCGGGCGGCTCAGACCACACACATAACCCGTGTCGCACCAGTTGT
Internal bar code:AATGGTGTTGGACCAGCTCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:198
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTCTTCGCTCTTGTGCA
Suggested primer 2:ACACACACCTTTCACCAGCA