Insertion junction: CLIP2.016122_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.016122
Chromosome:chromosome 6
Location:2961641
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g273650 (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCGTTATTAGCAAGCAGCTCGTTGTGGCGCCGTGACGCGCTGGCAGC
Internal bar code:AGAGAAGTATCTGATTTCAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAGGAACAACGCAAGAAA
Suggested primer 2:TGCTCACCCACATACGTGTC